Membrane Capacitance Changes due to Temperature Increase in Rat Cardiac Myocytes

Reconstruction of membrane current by deconvolution and its application to membrane capacitance measurements in cardiac myocytes

Correct detection of membrane currents under whole-cell patch-clamp conditions is limited by the transfer function of a recording system. The low-pass output filter of a recording amplifier alters the time course of membrane current and causes errors in relevant descriptors. To solve these problems, we developed a practical procedure for reconstruction of the time course of membrane currents ba...

Voltage-dependent membrane capacitance in rat pituitary nerve terminals due to gating currents.

We investigated the voltage dependence of membrane capacitance of pituitary nerve terminals in the whole-terminal patch-clamp configuration using a lock-in amplifier. Under conditions where secretion was abolished and voltage-gated channels were blocked or completely inactivated, changes in membrane potential still produced capacitance changes. In terminals with significant sodium currents, the...

Neonatal Rat Cardiac Myocytes

This study tested the hypothesis that the initiating mechanism is a major determinant of the response to calcium (Ca) accumulation in myocardium. Cultured neonatal rat ventriculocytes were exposed to Na', K+ pump inhibition with 1 mM ouabain and metabolic inhibition with 20 mM 2-deoxy--glucose and 1 mM cyanide (DOG-CN) for up to 2 h. Microspectrofluorometry of myocytes loaded with fura-2 showed...

Mitochondrial membrane potential in cardiac myocytes.

Mitochondria are involved in cellular functions that transcend the traditional role of these organelles as the energy factory of the cell. Their relative inaccessibility and the difficulties involved in attempts to study them in their natural environment -- the cytosol -- has delayed much of this understanding and they still have many secrets to yield. One of the relatively new fields in this r...

Membrane reactors: A way to increase efficiency